Genotyping and primer details


Genotyping and primer details for 234H08 - At1g50840
Line ID 234H08
Locus At1g50840 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:18843790 of line 234H08 Chr1:18843790
   
Insertion allele amplicon 8474 - 04jg
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 04jg: 5'-TGCTTAACTTTTCAATTGCGTATG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:18843487
   
wt amplicon (over InsPos) 21kj - 04jg
wt amplicon start Chr1:18843487
wt amplicon end Chr1:18844252
wt amplicon size 766 bp
Locus specific primer 04jg: 5'-TGCTTAACTTTTCAATTGCGTATG-3'
2nd locus specific primer 21kj: 5'-ATATTCCTTCCGTTAAAGGGCTTA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11