Genotyping and primer details


Genotyping and primer details for 236D09 - At5g06420
Line ID 236D09
Locus At5g06420 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:1962777 of line 236D09 Chr5:1962777
   
Insertion allele amplicon 8474 - 15uw
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 15uw: 5'-TAGTTTCCGTCTCTGTTTGGAGAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:1963225
   
wt amplicon (over InsPos) 41vl - 15uw
wt amplicon start Chr5:1962366
wt amplicon end Chr5:1963225
wt amplicon size 860 bp
Locus specific primer 15uw: 5'-TAGTTTCCGTCTCTGTTTGGAGAT-3'
2nd locus specific primer 41vl: 5'-AGGATTACAAGGAAACCGGATACT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11