Genotyping and primer details


Genotyping and primer details for 236D11 - At1g79510
Line ID 236D11
Locus At1g79510 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:29909101 of line 236D11 Chr1:29909101
   
Insertion allele amplicon 8474 - BW20
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer BW20: 5'-GGTGACCATATCCAAAACAGAAGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:29908424
   
wt amplicon (over InsPos) 47wn - BW20
wt amplicon start Chr1:29908424
wt amplicon end Chr1:29909831
wt amplicon size 1408 bp
Locus specific primer BW20: 5'-GGTGACCATATCCAAAACAGAAGT-3'
2nd locus specific primer 47wn: 5'-ATATCGCTAGTGTGGGCTTTCTTA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11