Genotyping and primer details


Genotyping and primer details for 237H05 - At5g28220
Line ID 237H05
Locus At5g28220 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:10191926 of line 237H05 Chr5:10191926
   
Insertion allele amplicon 8474 - 09kw
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 09kw: 5'-CGTGTGAAGCAACGTTTATAGTGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:10192217
   
wt amplicon (over InsPos) 10ls - 09kw
wt amplicon start Chr5:10191445
wt amplicon end Chr5:10192217
wt amplicon size 773 bp
Locus specific primer 09kw: 5'-CGTGTGAAGCAACGTTTATAGTGT-3'
2nd locus specific primer 10ls: 5'-TAGTGCCTTGGATATAGCCACTCT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11