Genotyping and primer details


Genotyping and primer details for 238B07 - At1g76170
Line ID 238B07
Locus At1g76170 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:28585870 of line 238B07 Chr1:28585870
   
Insertion allele amplicon 8474 - IN15
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer IN15: 5'-TGTTGACAGTTAAAGTTGTTTCGG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:28584944
   
Insertion allele amplicon 8474 - IN16
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer IN16: 5'-CATGAGATCCAGCTAATCACAAAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:28586512
   
wt amplicon (over InsPos) 04fa - IN15
wt amplicon start Chr1:28584944
wt amplicon end Chr1:28585915
wt amplicon size 972 bp
Locus specific primer IN15: 5'-TGTTGACAGTTAAAGTTGTTTCGG-3'
2nd locus specific primer 04fa: 5'-ATTGTTGGGAATCGTTTGTTTAAG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11