Genotyping and primer details


Genotyping and primer details for 241E09 - At3g18215
Line ID 241E09
Locus At3g18215 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:6241724 of line 241E09 Chr3:6241724
   
Insertion allele amplicon 8474 - 18hw
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 18hw: 5'-AGGAGAAGTAGAAAGCCCGTAATC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:6242176
   
wt amplicon (over InsPos) 20ix - 18hw
wt amplicon start Chr3:6241257
wt amplicon end Chr3:6242176
wt amplicon size 920 bp
Locus specific primer 18hw: 5'-AGGAGAAGTAGAAAGCCCGTAATC-3'
2nd locus specific primer 20ix: 5'-GTTCGTGATTTTAGGAATTGGGTA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11