Genotyping and primer details


Genotyping and primer details for 242H07 - At1g55840
Line ID 242H07
Locus At1g55840 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:20874325 of line 242H07 Chr1:20874325
   
Insertion allele amplicon 8474 - MP41
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer MP41: 5'-ACTTTTGCCCACAATTTTGTATTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:20873833
   
wt amplicon (over InsPos) 02bj - MP41
wt amplicon start Chr1:20873833
wt amplicon end Chr1:20874534
wt amplicon size 702 bp
Locus specific primer MP41: 5'-ACTTTTGCCCACAATTTTGTATTC-3'
2nd locus specific primer 02bj: 5'-TGGCTTTAAGAAAGCGTAACAAAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11