Genotyping and primer details


Genotyping and primer details for 244A10 - At3g25870
Line ID 244A10
Locus At3g25870 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:9464171 of line 244A10 Chr3:9464171
   
Insertion allele amplicon 8474 - 15uv
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 15uv: 5'-ACGTACGTACCGAATTTTAAACCA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:9464554
   
wt amplicon (over InsPos) 40vl - 15uv
wt amplicon start Chr3:9463795
wt amplicon end Chr3:9464554
wt amplicon size 760 bp
Locus specific primer 15uv: 5'-ACGTACGTACCGAATTTTAAACCA-3'
2nd locus specific primer 40vl: 5'-CCACATAACTCTCCAACCTCTGAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11