Genotyping and primer details


Genotyping and primer details for 245E05 - At1g03370
Line ID 245E05
Locus At1g03370 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:832144 of line 245E05 Chr1:832144
   
Insertion allele amplicon 8474 - MF07
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer MF07: 5'-GTTACCTTTCTGTTTTCTTGCCTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:832645
   
wt amplicon (over InsPos) 01li - MF07
wt amplicon start Chr1:831540
wt amplicon end Chr1:832645
wt amplicon size 1106 bp
Locus specific primer MF07: 5'-GTTACCTTTCTGTTTTCTTGCCTG-3'
2nd locus specific primer 01li: 5'-CCTCTACTTGTGCCTCTCCTTCTA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11