Genotyping and primer details


Genotyping and primer details for 245H11 - At2g40095
Line ID 245H11
Locus At2g40095 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:16743951 of line 245H11 Chr2:16743951
   
Insertion allele amplicon 8474 - 50rq
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 50rq: 5'-AACTATCGAGAGGACCGTATCTGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:16743537
   
wt amplicon (over InsPos) 80wa - 50rq
wt amplicon start Chr2:16743537
wt amplicon end Chr2:16744760
wt amplicon size 1224 bp
Locus specific primer 50rq: 5'-AACTATCGAGAGGACCGTATCTGA-3'
2nd locus specific primer 80wa: 5'-TTATCAGCAAAAGGGTTCTCAACT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11