Genotyping and primer details


Genotyping and primer details for 250D01 - At4g16090
Line ID 250D01
Locus At4g16090 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:9103370 of line 250D01 Chr4:9103370
   
Insertion allele amplicon 8474 - KB26
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer KB26: 5'-ATATCCTCTCCGAAGAAAGAATCC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:9103826
   
wt amplicon (over InsPos) 14sl - KB26
wt amplicon start Chr4:9103025
wt amplicon end Chr4:9103826
wt amplicon size 802 bp
Locus specific primer KB26: 5'-ATATCCTCTCCGAAGAAAGAATCC-3'
2nd locus specific primer 14sl: 5'-TGTGAAAACCCTTTTGTTTCTTTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11