Genotyping and primer details


Genotyping and primer details for 253C03 - At3g55060
Line ID 253C03
Locus At3g55060 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:20403308 of line 253C03 Chr3:20403308
   
Insertion allele amplicon 8474 - 16sj
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 16sj: 5'-TCACCACTAAGTTTATAAGCCCATTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:20403017
   
wt amplicon (over InsPos) 07sz - 16sj
wt amplicon start Chr3:20403017
wt amplicon end Chr3:20403763
wt amplicon size 747 bp
Locus specific primer 16sj: 5'-TCACCACTAAGTTTATAAGCCCATTT-3'
2nd locus specific primer 07sz: 5'-AAGTTTAGTGCGGGAGAAACTGTA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11