Genotyping and primer details


Genotyping and primer details for 256A08 - At3g54040
Line ID 256A08
Locus At3g54040 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:20014246 of line 256A08 Chr3:20014246
   
Insertion allele amplicon 8474 - 19gw
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 19gw: 5'-GGACTATTGCCCTAACATTCTTGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:20014737
   
wt amplicon (over InsPos) 09jz - 19gw
wt amplicon start Chr3:20013799
wt amplicon end Chr3:20014737
wt amplicon size 939 bp
Locus specific primer 19gw: 5'-GGACTATTGCCCTAACATTCTTGA-3'
2nd locus specific primer 09jz: 5'-AACGAACAAGACAACACACTGGTA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11