Genotyping and primer details


Genotyping and primer details for 256H07 - At1g55520
Line ID 256H07
Locus At1g55520 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:20727365 of line 256H07 Chr1:20727365
   
Insertion allele amplicon 8474 - 55fr
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 55fr: 5'-AAAAGCCTTCTCCTTTTTCAAATC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:20727948
   
wt amplicon (over InsPos) 09gi - 55fr
wt amplicon start Chr1:20726996
wt amplicon end Chr1:20727948
wt amplicon size 953 bp
Locus specific primer 55fr: 5'-AAAAGCCTTCTCCTTTTTCAAATC-3'
2nd locus specific primer 09gi: 5'-TGGAAGATTAGGCCATAAGAGAAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11