Genotyping and primer details


Genotyping and primer details for 259A08 - At1g42470
Line ID 259A08
Locus At1g42470 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:15930817 of line 259A08 Chr1:15930817
   
Insertion allele amplicon 8474 - MM60
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer MM60: 5'-TCTTAATTTCAGGTTCTGCTTGGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:15930412
   
wt amplicon (over InsPos) 02qy - MM60
wt amplicon start Chr1:15930412
wt amplicon end Chr1:15931184
wt amplicon size 773 bp
Locus specific primer MM60: 5'-TCTTAATTTCAGGTTCTGCTTGGT-3'
2nd locus specific primer 02qy: 5'-GACACAAGACAACTCCAATGAAAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11