Genotyping and primer details


Genotyping and primer details for 264F05 - At1g50140
Line ID 264F05
Locus At1g50140 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:18578580 of line 264F05 Chr1:18578580
   
Insertion allele amplicon 8474 - 17eq
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 17eq: 5'-GCAAATCAGAACATATCAGCAAAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:18578068
   
wt amplicon (over InsPos) 05fw - 17eq
wt amplicon start Chr1:18578068
wt amplicon end Chr1:18579167
wt amplicon size 1100 bp
Locus specific primer 17eq: 5'-GCAAATCAGAACATATCAGCAAAA-3'
2nd locus specific primer 05fw: 5'-CTACGACAATCGGTCTCTCTCTCT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11