Genotyping and primer details


Genotyping and primer details for 266A04 - At3g62680
Line ID 266A04
Locus At3g62680 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:23183755 of line 266A04 Chr3:23183755
   
Insertion allele amplicon 8474 - 14de
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 14de: 5'-TGTTTAGTAGACTCGTGTTGACGC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:23184150
   
wt amplicon (over InsPos) 35dy - 14de
wt amplicon start Chr3:23183273
wt amplicon end Chr3:23184150
wt amplicon size 878 bp
Locus specific primer 14de: 5'-TGTTTAGTAGACTCGTGTTGACGC-3'
2nd locus specific primer 35dy: 5'-CCATACGTACCAAAACCAACCTAC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11