Genotyping and primer details


Genotyping and primer details for 268H04 - At5g54710
Line ID 268H04
Locus At5g54710 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:22229144 of line 268H04 Chr5:22229144
   
Insertion allele amplicon 8474 - 18sj
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 18sj: 5'-TGGCAAGGACACTATTAGATGTTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:22229441
   
wt amplicon (over InsPos) 06sz - 18sj
wt amplicon start Chr5:22228821
wt amplicon end Chr5:22229441
wt amplicon size 621 bp
Locus specific primer 18sj: 5'-TGGCAAGGACACTATTAGATGTTG-3'
2nd locus specific primer 06sz: 5'-TTTTAGTAAACTGGAAGTCCCCAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11