Genotyping and primer details


Genotyping and primer details for 271D11 - At3g60100
Line ID 271D11
Locus At3g60100 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:22194779 of line 271D11 Chr3:22194779
   
Insertion allele amplicon 8474 - PA59
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer PA59: 5'-AGCAGAGCATAATTTTGTCAACCT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:22195365
   
wt amplicon (over InsPos) 21hf - PA59
wt amplicon start Chr3:22194452
wt amplicon end Chr3:22195365
wt amplicon size 914 bp
Locus specific primer PA59: 5'-AGCAGAGCATAATTTTGTCAACCT-3'
2nd locus specific primer 21hf: 5'-TAGCTTCATACGTTTATCGGAGGT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11