Genotyping and primer details


Genotyping and primer details for 272G09 - At3g12020
Line ID 272G09
Locus At3g12020 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:3831310 of line 272G09 Chr3:3831310
   
Insertion allele amplicon 8474 - 14kk
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 14kk: 5'-ATACACTGAAATTTGCTCATCGTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:3830905
   
wt amplicon (over InsPos) 07lb - 14kk
wt amplicon start Chr3:3830905
wt amplicon end Chr3:3831726
wt amplicon size 822 bp
Locus specific primer 14kk: 5'-ATACACTGAAATTTGCTCATCGTG-3'
2nd locus specific primer 07lb: 5'-GATCAAAGTGAACCATCATCAGAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11