Genotyping and primer details


Genotyping and primer details for 272H08 - At1g75140
Line ID 272H08
Locus At1g75140 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:28202242 of line 272H08 Chr1:28202242
   
Insertion allele amplicon 8474 - 09qw
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 09qw: 5'-GAGCCATCGAGAATCAACTAAAAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:28202766
   
wt amplicon (over InsPos) 05rm - 09qw
wt amplicon start Chr1:28201879
wt amplicon end Chr1:28202766
wt amplicon size 888 bp
Locus specific primer 09qw: 5'-GAGCCATCGAGAATCAACTAAAAC-3'
2nd locus specific primer 05rm: 5'-AAAGACCAAGACCAAGATGAAGAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11