Genotyping and primer details


Genotyping and primer details for 273E02 - At1g76540
Line ID 273E02
Locus At1g76540 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:28721657 of line 273E02 Chr1:28721657
   
Insertion allele amplicon 8474 - 14ns
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 14ns: 5'-ATCTGCTATTTTGAGCCTCATTGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:28721362
   
wt amplicon (over InsPos) 23oj - 14ns
wt amplicon start Chr1:28721362
wt amplicon end Chr1:28722036
wt amplicon size 675 bp
Locus specific primer 14ns: 5'-ATCTGCTATTTTGAGCCTCATTGT-3'
2nd locus specific primer 23oj: 5'-ATCTCGCTTGTTTTCCAGATTAGA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11