Genotyping and primer details


Genotyping and primer details for 275E02 - At3g56770
Line ID 275E02
Locus At3g56770 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:21030200 of line 275E02 Chr3:21030200
   
Insertion allele amplicon 8474 - PH68
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer PH68: 5'-ATTATGTGCCTCGTCTACTTCCAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:21030568
   
wt amplicon (over InsPos) 14rd - PH68
wt amplicon start Chr3:21029837
wt amplicon end Chr3:21030568
wt amplicon size 732 bp
Locus specific primer PH68: 5'-ATTATGTGCCTCGTCTACTTCCAC-3'
2nd locus specific primer 14rd: 5'-AAAAAGGGGCTTGTAAGCTTTTTA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11