Genotyping and primer details

Genotyping and primer details for 280E07 - At5g19010
Line ID 280E07
Locus At5g19010 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:6346711 of line 280E07 Chr5:6346711
Insertion allele amplicon 8474 - 33by
Locus specific primer 33by: 5'-GCCTGAGGTAACACATAAAACCAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:6346118
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11