Genotyping and primer details


Genotyping and primer details for 282D03 - At3g54980
Line ID 282D03
Locus At3g54980 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:20372313 of line 282D03 Chr3:20372313
   
Insertion allele amplicon 8474 - 21tm
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 21tm: 5'-AGGGTCTAAAGCCGAATAACTACA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:20371812
   
Insertion allele amplicon 8474 - 22tm
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 22tm: 5'-TCATCTCCTCAAACATCTTAACCA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:20372648
   
wt amplicon (over InsPos) 03tu - 21tm
wt amplicon start Chr3:20371812
wt amplicon end Chr3:20373235
wt amplicon size 1424 bp
Locus specific primer 21tm: 5'-AGGGTCTAAAGCCGAATAACTACA-3'
2nd locus specific primer 03tu: 5'-GCAGACGATATGCTAGAAGAATCA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11