Genotyping and primer details


Genotyping and primer details for 284A04 - At1g32100
Line ID 284A04
Locus At1g32100 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:11546686 of line 284A04 Chr1:11546686
   
Insertion allele amplicon 8474 - 15cv
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 15cv: 5'-CGAGGTAGGGTTCAATCAGTAGAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:11546267
   
wt amplicon (over InsPos) 38dy - 15cv
wt amplicon start Chr1:11546267
wt amplicon end Chr1:11547200
wt amplicon size 934 bp
Locus specific primer 15cv: 5'-CGAGGTAGGGTTCAATCAGTAGAG-3'
2nd locus specific primer 38dy: 5'-AATTCCTTACACTTACGTTGTGGG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11