Genotyping and primer details


Genotyping and primer details for 285F11 - At2g46380
Line ID 285F11
Locus At2g46380 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:19039437 of line 285F11 Chr2:19039437
   
Insertion allele amplicon 8474 - 03ih
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 03ih: 5'-TTCTTTTATTGTTGTCCCAAAACC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:19038887
   
wt amplicon (over InsPos) 12ji - 03ih
wt amplicon start Chr2:19038887
wt amplicon end Chr2:19039914
wt amplicon size 1028 bp
Locus specific primer 03ih: 5'-TTCTTTTATTGTTGTCCCAAAACC-3'
2nd locus specific primer 12ji: 5'-TATTACCCGAATCAACAACATTCA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11