Genotyping and primer details


Genotyping and primer details for 286D03 - At5g54920
Line ID 286D03
Locus At5g54920 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:22305549 of line 286D03 Chr5:22305549
   
Insertion allele amplicon 8474 - 11sf
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 11sf: 5'-TCATGTTTGATTTGTCTTAGGCTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:22305964
   
wt amplicon (over InsPos) 11ti - 11sf
wt amplicon start Chr5:22305266
wt amplicon end Chr5:22305964
wt amplicon size 699 bp
Locus specific primer 11sf: 5'-TCATGTTTGATTTGTCTTAGGCTG-3'
2nd locus specific primer 11ti: 5'-CTCTTTACTCCCCAACAGTTTCAG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11