Genotyping and primer details


Genotyping and primer details for 288C06 - At5g62130
Line ID 288C06
Locus At5g62130 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:24952651 of line 288C06 Chr5:24952651
   
Insertion allele amplicon 8474 - 18sa
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 18sa: 5'-TTCTTCACTTCCTCCTTCTCTCAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:24953232
   
wt amplicon (over InsPos) 28sq - 18sa
wt amplicon start Chr5:24952330
wt amplicon end Chr5:24953232
wt amplicon size 903 bp
Locus specific primer 18sa: 5'-TTCTTCACTTCCTCCTTCTCTCAC-3'
2nd locus specific primer 28sq: 5'-CCTACTACAGTGAAAATGAAGGGC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11