Genotyping and primer details


Genotyping and primer details for 290A03 - At2g44180
Line ID 290A03
Locus At2g44180 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:18269502 of line 290A03 Chr2:18269502
   
Insertion allele amplicon 8474 - 44fr
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 44fr: 5'-TGGATCAACAGGTAACAACAACAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:18269965
   
wt amplicon (over InsPos) 27go - 44fr
wt amplicon start Chr2:18268977
wt amplicon end Chr2:18269965
wt amplicon size 989 bp
Locus specific primer 44fr: 5'-TGGATCAACAGGTAACAACAACAT-3'
2nd locus specific primer 27go: 5'-AAACTTCCTTGTGGTTATGAATGG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11