Genotyping and primer details


Genotyping and primer details for 290B04 - At3g09540
Line ID 290B04
Locus At3g09540 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:2929491 of line 290B04 Chr3:2929491
   
Insertion allele amplicon 8474 - CL82
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer CL82: 5'-GGAAAAATTCGACAATCCTATCAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:2928948
   
wt amplicon (over InsPos) 17sq - CL82
wt amplicon start Chr3:2928948
wt amplicon end Chr3:2930004
wt amplicon size 1057 bp
Locus specific primer CL82: 5'-GGAAAAATTCGACAATCCTATCAA-3'
2nd locus specific primer 17sq: 5'-TTGATTGATATCACACGAGAAAGC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11