Genotyping and primer details


Genotyping and primer details for 290D05 - At5g44670
Line ID 290D05
Locus At5g44670 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:18020639 of line 290D05 Chr5:18020639
   
Insertion allele amplicon 8474 - 23fb
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 23fb: 5'-TCTGCACTTTAGCTACTCTCCTCC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:18021160
   
wt amplicon (over InsPos) 35gc - 23fb
wt amplicon start Chr5:18020212
wt amplicon end Chr5:18021160
wt amplicon size 949 bp
Locus specific primer 23fb: 5'-TCTGCACTTTAGCTACTCTCCTCC-3'
2nd locus specific primer 35gc: 5'-ATCCACTTCGCCATGAATCTATAC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11