Genotyping and primer details


Genotyping and primer details for 291A12 - At1g68110
Line ID 291A12
Locus At1g68110 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:25524698 of line 291A12 Chr1:25524698
   
Insertion allele amplicon 8474 - 22cy
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 22cy: 5'-AAGAACGGATCCATTTGTAGACAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:25525090
   
wt amplicon (over InsPos) 10em - 22cy
wt amplicon start Chr1:25524174
wt amplicon end Chr1:25525090
wt amplicon size 917 bp
Locus specific primer 22cy: 5'-AAGAACGGATCCATTTGTAGACAC-3'
2nd locus specific primer 10em: 5'-TCTTTTAGGGAGAGATTGAAAGCA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11