Genotyping and primer details


Genotyping and primer details for 292E02 - At2g45110
Line ID 292E02
Locus At2g45110 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:18599814 of line 292E02 Chr2:18599814
   
Insertion allele amplicon 8474 - CQ43
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer CQ43: 5'-GATTGGAGAGTTTGACCTCCATAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:18599360
   
wt amplicon (over InsPos) 06nv - CQ43
wt amplicon start Chr2:18599360
wt amplicon end Chr2:18600376
wt amplicon size 1017 bp
Locus specific primer CQ43: 5'-GATTGGAGAGTTTGACCTCCATAG-3'
2nd locus specific primer 06nv: 5'-TGTCCGATGCATACAACCTAATAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11