Genotyping and primer details


Genotyping and primer details for 292F01 - At5g14720
Line ID 292F01
Locus At5g14720 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:4752281 of line 292F01 Chr5:4752281
   
Insertion allele amplicon 8474 - 13dl
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 13dl: 5'-GTGATATGGCATTGATGATGTTTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:4752829
   
wt amplicon (over InsPos) 02ee - 13dl
wt amplicon start Chr5:4751934
wt amplicon end Chr5:4752829
wt amplicon size 896 bp
Locus specific primer 13dl: 5'-GTGATATGGCATTGATGATGTTTC-3'
2nd locus specific primer 02ee: 5'-AGTAGCGATAACAGGTTCCTCAAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11