Genotyping and primer details


Genotyping and primer details for 292G06 - At1g80120
Line ID 292G06
Locus At1g80120 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:30139222 of line 292G06 Chr1:30139222
   
Insertion allele amplicon 8474 - 16vf
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 16vf: 5'-GATTATCGAAGATCTCCTTACCCC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:30139674
   
wt amplicon (over InsPos) 47vx - 16vf
wt amplicon start Chr1:30138378
wt amplicon end Chr1:30139674
wt amplicon size 1297 bp
Locus specific primer 16vf: 5'-GATTATCGAAGATCTCCTTACCCC-3'
2nd locus specific primer 47vx: 5'-AAAAGGAGTGAAGGAGAAACAGTG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11