Genotyping and primer details


Genotyping and primer details for 293A04 - At5g44280
Line ID 293A04
Locus At5g44280 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:17838249 of line 293A04 Chr5:17838249
   
Insertion allele amplicon 8474 - AC23
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer AC23: 5'-AAACGTGAGAGTGTTTTGTGTTTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:17838762
   
wt amplicon (over InsPos) 42wn - AC23
wt amplicon start Chr5:17837525
wt amplicon end Chr5:17838762
wt amplicon size 1238 bp
Locus specific primer AC23: 5'-AAACGTGAGAGTGTTTTGTGTTTG-3'
2nd locus specific primer 42wn: 5'-AAAGCGTTTTAACAGCAACAATCT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11