Genotyping and primer details


Genotyping and primer details for 293F03 - At2g19210
Line ID 293F03
Locus At2g19210 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:8337895 of line 293F03 Chr2:8337895
   
Insertion allele amplicon 8474 - EQ15
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer EQ15: 5'-CGCTCACAATAGATTCGAATAACA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:8338431
   
Insertion allele amplicon 3144 - KH50
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer KH50: 5'-GTTGCATCAGTTAAGAATTCCACA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:8337588
   
wt amplicon (over InsPos) EQ15 - KH50
wt amplicon start Chr2:8337588
wt amplicon end Chr2:8338431
wt amplicon size 844 bp
Locus specific primer KH50: 5'-GTTGCATCAGTTAAGAATTCCACA-3'
2nd locus specific primer EQ15: 5'-CGCTCACAATAGATTCGAATAACA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11