Genotyping and primer details


Genotyping and primer details for 294B04 - At3g15355
Line ID 294B04
Locus At3g15355 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:5186415 of line 294B04 Chr3:5186415
   
Insertion allele amplicon 8474 - 11vi
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 11vi: 5'-TTGTCTCGATCCAAGGTCTAATCT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:5186055
   
wt amplicon (over InsPos) 34vq - 11vi
wt amplicon start Chr3:5186055
wt amplicon end Chr3:5187013
wt amplicon size 959 bp
Locus specific primer 11vi: 5'-TTGTCTCGATCCAAGGTCTAATCT-3'
2nd locus specific primer 34vq: 5'-AGTTGAAAGTACAACAACACGTCG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11