Genotyping and primer details


Genotyping and primer details for 295A05 - At5g50170
Line ID 295A05
Locus At5g50170 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:20421787 of line 295A05 Chr5:20421787
   
Insertion allele amplicon 8474 - 14dl
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 14dl: 5'-CATTAAGAACCTTCTCACCAGAGG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:20422109
   
wt amplicon (over InsPos) 03mf - 14dl
wt amplicon start Chr5:20421183
wt amplicon end Chr5:20422109
wt amplicon size 927 bp
Locus specific primer 14dl: 5'-CATTAAGAACCTTCTCACCAGAGG-3'
2nd locus specific primer 03mf: 5'-GATTTACCAGCGAAAGAGACATTC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11