Genotyping and primer details


Genotyping and primer details for 295F02 - At3g04450
Line ID 295F02
Locus At3g04450 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:1184441 of line 295F02 Chr3:1184441
   
Insertion allele amplicon 8474 - PF52
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer PF52: 5'-AAAGGCAAAACAAAAAGTGAAGAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:1184063
   
wt amplicon (over InsPos) 13li - PF52
wt amplicon start Chr3:1184063
wt amplicon end Chr3:1184772
wt amplicon size 710 bp
Locus specific primer PF52: 5'-AAAGGCAAAACAAAAAGTGAAGAA-3'
2nd locus specific primer 13li: 5'-CTTGGATGAAACCATAGTTCTGCT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11