Genotyping and primer details


Genotyping and primer details for 295G05 - At3g03150
Line ID 295G05
Locus At3g03150 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:728532 of line 295G05 Chr3:728532
   
Insertion allele amplicon 8474 - 14oz
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 14oz: 5'-TCTGGCTTTATAACTTCGTCAGGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:727902
   
wt amplicon (over InsPos) 40sq - 14oz
wt amplicon start Chr3:727902
wt amplicon end Chr3:728582
wt amplicon size 681 bp
Locus specific primer 14oz: 5'-TCTGGCTTTATAACTTCGTCAGGT-3'
2nd locus specific primer 40sq: 5'-TAGCTTGACCCAAAACTCAAATCT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11