Genotyping and primer details


Genotyping and primer details for 297H09 - At3g07990
Line ID 297H09
Locus At3g07990 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:2552679 of line 297H09 Chr3:2552679
   
Insertion allele amplicon 8474 - BV06
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer BV06: 5'-CACACAACAGACAACAACACTCAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:2552085
   
Insertion allele amplicon 8474 - BV07
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer BV07: 5'-GGTAGAAACTGAAACCTGCATAGC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:2553300
   
wt amplicon (over InsPos) BV07 - BV06
wt amplicon start Chr3:2552085
wt amplicon end Chr3:2553300
wt amplicon size 1216 bp
Locus specific primer BV06: 5'-CACACAACAGACAACAACACTCAG-3'
2nd locus specific primer BV07: 5'-GGTAGAAACTGAAACCTGCATAGC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11