Genotyping and primer details


Genotyping and primer details for 298B11 - At3g62240
Line ID 298B11
Locus At3g62240 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:23035377 of line 298B11 Chr3:23035377
   
Insertion allele amplicon 8474 - 40qa
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 40qa: 5'-AGAAAGAGGAGGAAAAGCAGATTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:23034789
   
wt amplicon (over InsPos) 04vu - 40qa
wt amplicon start Chr3:23034789
wt amplicon end Chr3:23036202
wt amplicon size 1414 bp
Locus specific primer 40qa: 5'-AGAAAGAGGAGGAAAAGCAGATTC-3'
2nd locus specific primer 04vu: 5'-ACACTCAGGCTTTCTTTGATGACT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11