Genotyping and primer details


Genotyping and primer details for 300D09 - At3g60510
Line ID 300D09
Locus At3g60510 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:22358084 of line 300D09 Chr3:22358084
   
Insertion allele amplicon 8474 - 17kh
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 17kh: 5'-AATTAGTCGGACAGCTGGATTCTA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:22358664
   
wt amplicon (over InsPos) 01le - 17kh
wt amplicon start Chr3:22357572
wt amplicon end Chr3:22358664
wt amplicon size 1093 bp
Locus specific primer 17kh: 5'-AATTAGTCGGACAGCTGGATTCTA-3'
2nd locus specific primer 01le: 5'-GTTGGATTCGTTTTAAAGAAGCTG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11