Genotyping and primer details


Genotyping and primer details for 308C04 - At2g40400
Line ID 308C04
Locus At2g40400 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:16871864 of line 308C04 Chr2:16871864
   
Insertion allele amplicon 8474 - AB56
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer AB56: 5'-ATCCATACTTGCCTCTTCAGTTGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:16872551
   
wt amplicon (over InsPos) 07lt - AB56
wt amplicon start Chr2:16871203
wt amplicon end Chr2:16872551
wt amplicon size 1349 bp
Locus specific primer AB56: 5'-ATCCATACTTGCCTCTTCAGTTGT-3'
2nd locus specific primer 07lt: 5'-CTTTGATTTGGAGCAATATCCTCT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11