Genotyping and primer details


Genotyping and primer details for 308G07 - At1g77460
Line ID 308G07
Locus At1g77460 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:29108879 of line 308G07 Chr1:29108879
   
Insertion allele amplicon 8474 - 34by
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 34by: 5'-GTAAATCTATCCCGCTGCTTGTAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:29108355
   
wt amplicon (over InsPos) 16dd - 34by
wt amplicon start Chr1:29108355
wt amplicon end Chr1:29109280
wt amplicon size 926 bp
Locus specific primer 34by: 5'-GTAAATCTATCCCGCTGCTTGTAG-3'
2nd locus specific primer 16dd: 5'-AGGTAGTAGCTCAAGACATCGCTC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11