Genotyping and primer details


Genotyping and primer details for 310B09 - At3g17800
Line ID 310B09
Locus At3g17800 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:6091406 of line 310B09 Chr3:6091406
   
Insertion allele amplicon 8474 - 34ah
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 34ah: 5'-CTGAACTCTGTTGCACAGATAAGC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:6091808
   
Insertion allele amplicon 8474 - AB60
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer AB60: 5'-TCAATCCCAAGCTACTGAAATTCT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:6090834
   
wt amplicon (over InsPos) 34ah - AB60
wt amplicon start Chr3:6090834
wt amplicon end Chr3:6091808
wt amplicon size 975 bp
Locus specific primer AB60: 5'-TCAATCCCAAGCTACTGAAATTCT-3'
2nd locus specific primer 34ah: 5'-CTGAACTCTGTTGCACAGATAAGC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11