Genotyping and primer details


Genotyping and primer details for 312E07 - At5g65340
Line ID 312E07
Locus At5g65340 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:26114117 of line 312E07 Chr5:26114117
   
Insertion allele amplicon 8474 - FV61
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer FV61: 5'-TCCTTGTGCTAGAACACACAAAAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:26113651
   
Insertion allele amplicon 8474 - FV62
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer FV62: 5'-TCCGCGTACAATAATCCTTTTAAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:26114776
   
wt amplicon (over InsPos) FV61 - FV62
wt amplicon start Chr5:26113651
wt amplicon end Chr5:26114776
wt amplicon size 1125 bp
Locus specific primer FV62: 5'-TCCGCGTACAATAATCCTTTTAAC-3'
2nd locus specific primer FV61: 5'-TCCTTGTGCTAGAACACACAAAAC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11