Genotyping and primer details


Genotyping and primer details for 312H09 - At2g45160
Line ID 312H09
Locus At2g45160 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:18618138 of line 312H09 Chr2:18618138
   
Insertion allele amplicon 8474 - 12lu
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 12lu: 5'-AAATTAACAGTCTTTGCTCCTCCA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:18618778
   
wt amplicon (over InsPos) 05mo - 12lu
wt amplicon start Chr2:18617805
wt amplicon end Chr2:18618778
wt amplicon size 974 bp
Locus specific primer 12lu: 5'-AAATTAACAGTCTTTGCTCCTCCA-3'
2nd locus specific primer 05mo: 5'-GGATCTTTACCAGAATTTTCCACA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11